カタツムリAchatinaFulicaの粘液は、人間の軟骨細胞にプラスの効果があります

この研究は、インビトロでの変形性関節症および軟骨組織修復における潜在的な治療的修復剤としてのAchatina fulica粘液の新規使用を報告します。. カタツムリの粘液が分離されました, 滅菌済み, FTIRを使用して特性評価, XPS, レオロジー, およびLC-MS/MS. GAG, 砂糖, フェノール, タンパク質含有量は標準アッセイを使用して推定されました. The LC–MS/MS identified 6-gingerol and some other small molecules. The effects of the sterilized mucus were studied on human chondrocytes using the C28/I2 cell as a model for the in vitro assays. The MTT assay indicates that mucus extracted from the pedal of A. fulica is biocompatible with the cells up to a concentration of 50 μg/mL. The mucus promoted cell migration and proliferation and completely closed the wound within 72 h, as indicated in the in vitro scratch assay. In addition, the snail mucus reduced apoptosis significantly (p < 0.05) in the treated cells by 74.6%. It preserved the cytoskeletal integrity of the C28/I2 cells, attributed mainly to GAGs and 6-gingerol content of the mucus. In conclusion, this present study suggests that GAGs and 6-gingerol conferred wound-healing and antiapoptotic properties on the mucus secretion from A. fulica and can be explored for therapeutic repair and cartilage tissue engineering.

Victor A. and Ashok M. Raichur